Insect resistance. Hessian fly.
Protocol contributed by Christie Williams and Herb Ohm
The H31 gene was identified in an accession of Triticum turgidum Desf. (durum wheat), CI3984, from Tunisia. Williams et al. (1) transferred this gene to hexaploid wheat, the resulting line was P921696 (PI 633876), a soft red winter wheat. H31 is located near the terminus of chromosome arm 5BS, this is the first Hessian fly resistance gene reported on that arm. The two other genes conferring resistance to biotype L, H9 and H13, were mapped on chromosome arms 5AL and 6DL respectively. Because of the diverse locations of the three loci and the availability of molecular markers for all of them, they are a convenient target for pyramiding.
PCR products were visualized on a standard 1% agarose gel with etidium bromide staining.
Conditions presented here should be considered only as a starting point of the PCR optimization for individual laboratories.
1. Phenotypic assessment and mapped markers for H31, a new wheat gene conferring resistance to Hessian fly (Diptera: Cecidomyiidae). Williams CE, Collier N, Sardesai CC, Ohm HW, Cambron SE. In: Theoretical and Applied Genetics, 2003, 107:1516-1523. DOI:10.1007/s00122-003-1393-y