Disease resistance. Leaf rust.
Lr39
Contributed by Gina Brown-Guedira and Suhkwinder Singh
Microsatellite marker for Lr39
GDM35 primer sequences and amplification conditions are as in Pestsova et al. 2000 (Genome 43(4):689-697).
Primers sequences:
GDM35-L 5'- CCT GCT CTG CCC TAG ATA CG -3' GDM35-R 5'- ATG TGA ATG TGA TGC ATG CA -3'
Final concentrations of the reagents used in the PCR amplification (volume: 25 µl)
- 50 ng genomic DNA
- 12.5 pmol each primer
- 0.2 mM each dNTP
- 0.625 U Taq
- 1x PCR buffer
- 2 mM MgCl2
PCR cycling:
- 4 min at 94°C
- 30 cycles of 30s at 94°C, 30s at 55°C, 30s at 72°C
- Final extension for 5 min at 72°C.
PCR products are resolved on 2.3% SFR high resolution agarose gels.
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| Figure 2. Amplification of wheat microsatellite marker GDM35 in a population of F3 lines from the cross KS89WGRC10 x TAM 107. A= homozygous resistant lines, B = homozygous susceptible lines and H =segregating. |