Disease resistance. Leaf rust.
Lr46 - Yr29
Microsatellite locus Xwmc44 maps 5-15 cM proximal to Lr46.Primers sequences:
WMC44-forward 5'- GGT CTT CTG GGC TTT GAT CCT G -3' WMC44-reverse 5'- GTT GCT AGG GAC CCG TAG TGG -3'PCR conditions:
At the recommended annealing temperature, 61°C, polymorphic products are observed with either agarose or polyacrylamide. The expected product for Chinese Spring is a band of 242 bases.
Microsatellite locus Xgwm259 maps approximately 20 cM distal to Lr46 and can be used as a flanking marker together with Xwmc44.Primers sequences:
WMS259-forward 5'- AGG GAA AAG ACA TCT TTT TTT TC -3' WMS259-reverse 5'- CGA CCG ACT TCG GGT TC -3'PCR conditions:
Microsatellite locus Xbarc80 maps 10-11 cM distal to Xgwm259, and can be used as an alternative distal marker.Primers sequences:
BARC80-forward 5'- GCG AAT TAG CAT CTG CAT CTG TTT GAG -3' BARC80-reverse 5'- CGG TCA ACC AAC TAC TGC ACA AC -3'PCR conditions:
Conditions presented here should be consider only as a starting point of the PCR optimization for individual laboratories.