Disease resistance. Leaf rust.

Lr46 - Yr29

Marker Xwmc44

Microsatellite locus Xwmc44 maps 5-15 cM proximal to Lr46.

Primers sequences:

   WMC44-forward   5'- GGT CTT CTG GGC TTT GAT CCT G  -3'
   WMC44-reverse   5'- GTT GCT AGG GAC CCG TAG TGG -3'

PCR conditions:

At the recommended annealing temperature, 61°C, polymorphic products are observed with either agarose or polyacrylamide. The expected product for Chinese Spring is a band of 242 bases.

Denaturing step: 94°C, 3 min
Amplification step (45 cycles):
- 94°C, 30 s
- 61°C, 30 s
- 72°C, 90 s
Extension step: 72°C, 10 min

Expected products:

Xwmc44

Microsatellite marker Xwmc44: The cyan arrowhead indicates the Pavon 76 allele. The allele is not diagnostic of Lr46, since numerous varieties without Lr46 have WMC44 products of similar sizes. Green, red and violet arrowheads indicate different alleles. M, size markers.

Marker Xgwm259

Microsatellite locus Xgwm259 maps approximately 20 cM distal to Lr46 and can be used as a flanking marker together with Xwmc44.

Primers sequences:

   WMS259-forward   5'- AGG GAA AAG ACA TCT TTT TTT TC -3'
   WMS259-reverse   5'- CGA CCG ACT TCG GGT TC -3'

PCR conditions:

Denaturing step: 94°C, 3 min
Amplification step (45 cycles):
- 94°C, 30 s
- 56°C, 30 s
- 72°C, 90 s
Extension step: 72°C, 10 min

Expected products:

Microsatellite marker Xgwm259: The orange arrow shows the band observed in Pavon 76, which carries Lr46. CS, Chine Spring; M, size markers.

Marker Xbarc80

Microsatellite locus Xbarc80 maps 10-11 cM distal to Xgwm259, and can be used as an alternative distal marker.

Primers sequences:

   BARC80-forward   5'- GCG AAT TAG CAT CTG CAT CTG TTT GAG -3'
   BARC80-reverse   5'- CGG TCA ACC AAC TAC TGC ACA AC -3'

PCR conditions:

Denaturing step: 94°C, 3 min
Amplification step (45 cycles):
- 94°C, 30 s
- 50°C, 30 s
- 72°C, 60 s
Extension step: 72°C, 10 min

Expected products:

Microsatellite marker Xbarc80: The orange arrow shows the band observed in Pavon 76, which carries Lr46. CS, Chine Spring; M, size markers.

Conditions presented here should be consider only as a starting point of the PCR optimization for individual laboratories.

[Back to top] [Back to Lr46 main page]