Disease resistance. Leaf Rust Resistance.

Lr51

1. Transfer of leaf rust resistance from Aegilops speltoides to Triticum aestivum. Dvorak J. In: Canadian Journal of Genetics and Cytology,1977, 19:133-141.

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2. PCR markers for Triticum speltoides leaf rust resistance gene Lr51 and their use to develop isogenic hard red spring wheat lines. Helguera M, Vanzetti L, Soria M, Khan IA, Kolmer J, Dubcovsky J. In: Crop Science. In press.

New leaf rust resistance genes are needed in wheat to provide additional sources of resistance to the highly variable and dynamic leaf rust pathogen Puccinia triticina. Leaf rust resistance gene Lr51, located within a segment of Triticum speltoides chromosome 1S translocated to the long arm of chromosome 1B of bread wheat, is resistant to the current predominant races of leaf rust in the U.S. The objectives of this study were to determine the genetic length of the translocated 1S segment, develop a PCR marker for Lr51 and use this marker to generate isogenic lines for this gene. Characterization of two translocation lines (F-7-3 and F-7-12) with 10 molecular markers indicated that F-7-3 has an interstitial T. speltoides chromosome segment of 14 to 32-cM long including loci XAga7 and Xmwg710, whereas line F-7-12 has a complex series of translocations among chromosomes of homoeologous group 1. Based on the DNA sequence of the A, B, D and S-alleles of the XAga7 locus, we designed a Cleavage Amplified Polymorphic Sequence (CAPS) marker for the S genome allele. Primers S30-13L and AGA7-759R preferentially amplified the XAga7 1S (819-bp) and 1B alleles (783-bp). These amplification products can be separated in agarose gels after digestion with Pst I or BamH I restriction enzymes. This CAPS marker was validated in a collection of 33 common wheat cultivars, and was used to develop three pairs of hard red spring isogenic lines using the F-7-3 line as a donor parent. These isogenic lines will be valuable for future assessment of the effect of this chromosome introgression on agronomic performance and end-use quality.

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3. Catalogue of Gene Symbols for Wheat. McIntosh RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers WJ, Appels R. In: Proceedings of the 10th International Wheat Genetics Symposium. Volume 4. Instituto Sperimentale per la Cerealicoltura, Rome, Paestum, Italy.2003

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