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Virus resistance. Wheat Streak Mosaic Virus (WSMV)

Wsm1

Background information

The gene Wsm1, encoding resistance to WSMV, originates from a translocation of Agropyron intermedium into wheat (1). Among the first wheat lines containing  Wsm1 was CI 17884, which has the short arm of  chromosome 4D replaced by the short arm of chromosome 4 from A. intermedium. Further germplasm  improvements were achieved by backcrossing CI 17884 into cultivar Karl to produce KS93WGRC27 (2), this line or lines derived from it are used to incorporate Wsm1 into wheat cultivars.

Methods

The translocated segment of A. intermedium containing the Wsm1 gene can be detected using the set of PCR primers STSJ15 (Talber et al, 1996). Since these primers will not amplify if the segment is absent, the set of primers G43 can be used as a positive control of the PCR reactions (see protocol).

Current backcrossing programs for WSMV resistance gene within the project

Donor Recurrent Parent Laboratory code Market class
KS98HW189-3 CO99508 CO HRW
KS98HW189-3 CO970547 CO HRW
KS98HW189-3 Above (CO980894) CO HRW
KS98HW189-3 Avalanche (CO940611) CO HWW
KS98HW189-3 Lakin CO HWW
KS93WGRC27 KS97P0630-4-5 KS HRW
KS93WGRC27 Jagger KS HRW
KS93WGRC27 Trego KS HWW
KS93WGRC27 Lakin KS HWW
KS93WGRC27/McNeal MT9929 MT HRS
KS93WGRC27/McNeal MT9874 MT HRS
KS93WGRC27/McNeal Hank MT HRS
KS93WGRC27 McNeal MT HRS
KS93WGRC27/McNeal Scholar MT HRS
KS93WGRC27/McNeal MTHW9710 MT HWS
adv. lines from KS93WGRC27 Alliance NE HRW
adv. lines from KS93WGRC27 Wahoo NE HRW
adv. lines from KS93WGRC27 Millennium NE HRW
adv. lines from KS93WGRC27 Culver NE HRW
adv. lines from KS93WGRC27 Pronghorn NE HRW

Laboratory codes: CO, Colorado State University; KS, Kansas State University; MT, Montana State University; NE, University of Nebraska

Additional information. Field testing

Sharp et al (4) reported a yield penalty in the lines carrying the translocation. But Wsm1 is the best source of WSMV resistance, compared to adapted cultivars or transgenic lines for the viral replicase or coat protein. Baley et al. (5) developed populations segregating for  WSMV resistance and showed that noninoculated  resistant lines had a slightly lower but significant decrease in yield compared to noninoculated susceptible lines. However, resistant lines presented only a 5% reduction in yield when inoculated with WSMV, compared to a 32% reduction for the susceptible lines.

References

1. Identification of alien chromatin specifying resistance to wheat streak mosaic and greenbug in wheat germ plasm by C-banding and in situ hybridization. Friebe, B.; Mukai, Y.; Dhaliwal, H. S.; Martin, T. J.; Gill, B. S. In: Theoretical and Applied Genetics, 1991. 81(3):381-389 [abstract]

2. Registration of KS93WGRC27 wheat streak mosaic virus resistant T4DL.4Ai#2S wheat germplasm Gill, B. S.; Friebe, B.; Wilson, D. L.; Martin, T. J.; Cox, T. S. In: Crop Science, 1995. 35(4):1236-1237

3. Development of PCR markers linked to resistance to wheat streak mosaic virus in wheat. Talbert, L. E.; Bruckner, P. L.; Smith, L. Y.; Sears, R.; Martin, T. J.In: Theoretical and Applied Genetics, 1996. 93 (3):463-467  [abstract]

4. Field Evaluation of Transgenic and Classical Sources of Wheat streak mosaic virus Resistance.  Sharp, G. L.; Martin,  J. M. ; Lanning, S. P.; Blake,  N. K. ; Brey, C. W. ; Sivamani, E.; Qu,  R.; Talbert, L. E.  In: Crop Science, 2002. 42(1):105-110 [abstract]

5. Agronomic and End-Use Qualities of Wheat streak mosaic virus Resistant Spring Wheat. Baley,  G. J.; Talbert, L. E. ; Martin,  J. M.; Young, M. J. ; Habernicht, D. K. ; Kushnak, G. D. , Berg, J. E. ; Lanning, S. P. ; Bruckner, P. L. In: Crop Science, 2001. 41(6):1779-1784 [abstract]

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