Virus resistance. Wheat Streak Mosaic Virus (WSMV)
Detection of the Agropyron intermedium segment carrying Wsm1
This protocol is based on the information presented by Jamie Sherman during the coordination meeting at PAGX, you can also see Ref. 3
Sequences of the J15 primers (Wsm1) :
This sequence-tagged-site (STS) PCR primers are specific for the A. intermedium segment containing the Wsm1 gene
J15L: 5'- GTA GCA GGG GAA GCT GAA GA -3'
J15R: 5'- CCG AGC TCA CAC GCT AAT TT -3'
Sequences of the G43 primers :
These primers are used as positive control of the PCR reaction (J15 will not amplify when the A. intermedium segment is absent)
G43L: 5'- GGC GCA TGC ACC AAA ATG TT -3'
G43R: 5'- ACC TTG TCG TGC ATA GGA AC -3'
PCR amplification conditions:
Final volume: 50 ul
Final concentrations of the products used in the PCR reaction:
Expected PCR product sizes: