Disease resistance. Wheat Stem Sawfly (WSS).
Solid stem locus
Contributed by Luther Talbert (email@example.com )
Microsatellite marker Xgwm340
Due to the to the quantitative nature of the solid stem phenotype it is not possible to estimate an exact distance, but it was estimated that the Xgwm340 is located within 2 cM of the solid stem locus, Qss.msub-3BL. So far, no other flanking marker could be identified close enough to be of practical use for breedingPrimers sequences:
WMS340-L 5'- GCA ATC TTT TTT CTG ACC ACG -3' WMS340-R 5'- ACG AGG CAA GAA CAC ACA TG -3'PCR conditions:
Total volume: 25 µlExpected products:
Conditions presented here should be consider only as a starting point of the PCR optimization for individual laboratories.