Disease resistance. Insensitivity to the toxins produced by Pyrenophora tritici-repentis and Stagonospora nodorum.
Contributed by Justin Faris (firstname.lastname@example.org)
Microsatellites markers for Tsn1
Marker: Xfcp1Final concentrations of the reagents used in the PCR amplificationForward primer: 5'- ATA ACT CCG TCA CGA CCA CCT CCT CTC AAG -3' Reverse primer: 5'- CAG TCT GAA AAC GCC ATA CCC G -3'
Total 25 ulPCR conditions:
Conditions presented here should be consider only as a starting point of the PCR optimization for individual laboratories.